Improved Localisation and Reconstruction in a Single Molecule Localisation Microscope to Enable Super-resolution Imaging with Lowered Resource Pre-requisites

Abstract

Super-resolution microscopy techniques overcome the diffraction limit of conventional optical microscopes, enabling the visualisation of nanometre-scale structures using visible light. Among these, Single Molecule Localisation Microscopy (SMLM) including techniques like PALM, STORM, and PAINT allows super-resolution imaging using minimally modified fluorescence microscopes. By temporally separating the stochastic emission of fluorophores and localising them computationally, SMLM reconstructs images with nanometre-level precision.