Therapeutic and Diagnostic Potential of Rotavirus A Capsid Proteins
| dc.contributor.author | Kuri, Pooja Rani | |
| dc.date.accessioned | 2025-04-16T06:10:04Z | |
| dc.date.available | 2025-04-16T06:10:04Z | |
| dc.date.issued | 2024 | |
| dc.description | Supervisor: Goswami, Pranab | |
| dc.description.abstract | Rotavirus, a primary contributor to severe cases of infantile gastroenteritis on a global scale, causes significant morbidity and mortality in the under-five population, particularly in middle to low-income countries, including India. Effective management of rotavirus disease encompasses both suitable diagnostic strategies and effective therapeutic interventions. Implementation of WHO-approved live-attenuated vaccines in certain regions of the world brought about a significant reduction in rotavirus-led gastroenteritis hospitalizations. However, they are linked to susceptibility to intussusception and exhibit low efficacy, primarily attributed to the high genetic diversity of rotavirus, varying over time and across different geographic regions. Herein, molecular data on Indian rotavirus A (RVA) has been reviewed through phylogenetic analysis, revealing G1P[8] to be the prevalent strain of RVA in India. The conserved sequences of rotavirus capsid proteins VP7, VP4 and VP6 across G1P[8] strain were used to examine helper T lymphocyte, cytotoxic T lymphocyte and linear B cell epitopes. Twenty epitopes were identified after evaluation of factors such as antigenicity, non-allergenicity, non-toxicity, and stability. These epitopes were then interconnected using suitable linkers and an N-terminal beta defensin adjuvant. The in silico designed vaccine exhibited structural stability and interactions with integrins (αvβ3 and αIIbβ3) and toll-like receptors (TLR2 and TLR4) indicated by docking and normal mode analyses. The immune simulation profile of the designed RVA multiepitope vaccine exhibited its potential to trigger humoral as well as cell-mediated immunity, indicating that it is a promising immunogen. These computational findings indicate potential efficacy of the designed vaccine against rotavirus infection. | |
| dc.identifier.other | ROLL NO.176106028 | |
| dc.identifier.uri | https://gyan.iitg.ac.in/handle/123456789/2879 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | TH-3543 | |
| dc.title | Therapeutic and Diagnostic Potential of Rotavirus A Capsid Proteins | |
| dc.type | Thesis |
Files
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed to upon submission
- Description: