Studies on Nanomat erials and A biomaterial as cholesterol oxidase-Immobilization supports for Developing cholesterel biosensor
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Date
2011
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Abstract
This study aims at fabricating different kinds of cholesterol biosensors where cholesterol oxidase (ChOx) from Pseudomonas fluorescens has been used as the biorecognition element. Preliminary characterization of the ChOx has been carried out in terms of molecular weight, kinetic parameters, optimum working parameters etc. The molecular weight of the ChOx was found to be ~57 kDa. The N-terminal sequencing of the P. fluorescens ChOx was carried out and the obtained N-terminal sequence was found to be similar to the N-terminal sequence of ChOx protein from Burkholderia cepacia. Product analysis demonstrated that P. fluorescens ChOx produces 6D-hydroperoxycholest-4-en-3- one (HCEO) upon cholesterol oxidation. This P. fluorescens ChOx was first covalently immobilized onto woven silk-mat using N-ethyl-ND-(3-dimethylaminopropyl) carbodimide (EDC) and N-hydroxysuccinimide (NHS) ligand chemistry. bioelectrode. The enzyme loading and the 0.046 U cm-2 silk-mat and 70 %, respectively..
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Supervisor: Pranab Goswami
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BIOSCIENCES AND BIOENGINEERING