Isolation and characterization of mesenchymal stem cells from ocular adipose tissue and extra ocular muscle
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Adult stem cells are cells capable to self-renew and are multipotent in nature. Mesenchymal stem cells can be isolated in vitro originally from the bone marrow but off-late they have also been isolated from various fetal and adult tissues such as the adipose, limbus, skin, intestinal tissue, various part of the eye, umbilical cord blood, umbilical cord, Wharton’s jelly, and even placenta of the mother and the fetus. They expressed and are identified by an array of surface markers, can differentiate into mesodermal lineage owing to their origin and can on induction also differentiate into neural cells, especially into neuronal cells, and hepatic cells. They can escape the immunogenic effects of the host environment. Owing to these properties mesenchymal stem cells are proposed to be good candidates for cell therapy for neurological disorders.OAT derived mesenchymal progenitors had been previously reported by (Korn, Kikkawa, & Hicok, 2009). We isolated OAT-MSC from central and medial orbital tissue of the upper eyelid. The MSC derived from both the tissues were spindle shaped and adherent in nature. They expressed mesenchymal markers on their cell surface and differentiated into adipocytes, osteocytes and chondrocytes. They were differentiated into neuronal cells and differentiation was confirmed by the expression of NGFR. We found out that central OAT-MSC had more capacity to differentiate into adipocytes and osteocytes compared to medial OAT-MSC.
Supervisor: Bithiah Grace Jaganathan
BIOSCIENCES AND BIOENGINEERING