Studies on immobilized lipase and biosurfactant for hydrolysis and transesterification of sunflower oil

Simple item page
dc.contributor.authorChatterjee, Sushovan
dc.date.accessioned2020-08-13T07:24:37Z
dc.date.accessioned2023-10-26T10:21:58Z
dc.date.available2020-08-13T07:24:37Z
dc.date.available2023-10-26T10:21:58Z
dc.date.issued2012
dc.descriptionSupervisor: Goswami, Pranaben_US
dc.description.abstractThe principal objective of this work is immobilization of lipase and application of the immobilized lipase for hydrolysis and transesterification of sunflower oil. Lipase was isolated from the culture supernatant of Pseudomonas aeruginosa PG01. A high activity (4.1+- 0.05 U/ml) in the supernatant was detected when sunflower oil (2g/l) was used as the sole substrate. A significant production of lipase in burnt oil was observed, which was about 75% of the activity obtained when sunflower oil was used as the substrate. The extracellular lipase was successfully fractionated from the culture supernatant by ammonium- sulfate fractionating method, which was further purified by DEAS- sepharose anion exchange chromatography. The specific activity of the purified lipase was increased to 2.59 fold. The molecular weight of the DEAS sepharose filtered purified pseudomonas lipase was estimated by SDS-PAGE was found to be 55 kDa. The optimum temperature and pH of the isolated lipase was 30oC and 7, respectively. The t1/2 of the lipase at 4C and pH 7 was nearly 23 months. The stability of the powdered lipase diminished steadily with the increasing temperature from 20o C to 80o C with t1/2 of 62 days and 82 min at 20o C and 80o C respectively.en_US
dc.identifier.otherROLL NO.05615101
dc.identifier.urihttps://gyan.iitg.ac.in/handle/123456789/1575
dc.language.isoenen_US
dc.relation.ispartofseriesTH-1854;
dc.subjectENERGY SCIENCE AND ENGINEERINGen_US
dc.titleStudies on immobilized lipase and biosurfactant for hydrolysis and transesterification of sunflower oilen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Abstract-TH-1854_Chatterjee.pdf
Size:
4.07 MB
Format:
Adobe Portable Document Format
Description:
Abstract
Download
No Thumbnail Available
Name:
TH-1854_Chatterjee.pdf
Size:
250.53 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Plain Text
Description:
Download
Collections
PhD Theses (Energy Science and Engineering)