Phytochemical Conjugated Gold Nanoparticles for the Treatment of Triple-Negative Breast Cancer

Simple item page
dc.contributor.authorUnnikrishnan, Jyothsna
dc.date.accessioned2025-01-10T11:57:35Z
dc.date.available2025-01-10T11:57:35Z
dc.date.issued2023
dc.descriptionSupervisor: Satpati, Priyadarshi
dc.description.abstractOff late by 2020, breast cancer (BC) has superseded lung cancer incidences globally. Being the most aggressive subtype of BC, triple-negative breast cancer (TNBC) is difficult to tackle with conventional treatment approaches. Even though side effects restrain the use of contemporary methods; surgery, chemotherapy and radiation remain in the mainstream. At the beginning of the 19th century, improvements in organic chemistry unveiled active compounds from plants called phytochemicals, found to have excellent therapeutic efficacy against cancer. Nevertheless, low bioavailability and hydrophobicity limit the potential of these plant-derived compounds. It is well evident that nanotechnology tools could effectively bypass these limitations. Therefore, in the current study, phytochemical conjugated gold nanoparticles (AuNp) were prepared to target TNBC cells via EPR effect passively. Phytochemicals such as ethyl ferulate (EF), ellagic acid (EA), coronarin D (CD) and epoxy azadiradione (EAZD) were used to reduce gold chloride into AuNps. Formation of EF-AuNp, EA-AuNp, CD-AuNp and EAZD-AuNp were confirmed by UV-visible spectroscopy, XRD, EDX, TEM and FT-IR spectra. Our in-vitro analysis using MTT assay, colony-formation assay, PI-FACS and live and dead assay evidenced the anti-cancer potency of these phyo-nano complexes. Conclusively, EAZD-AuNp induced superior antiproliferative and cytotoxic effects in MDA-MB-231 cells than other nanoparticles. Hence, it is worthwhile to proceed with the in-vivo studies and the molecular pathway analysis of this meritorious compound which would help this drug journey to the clinic.
dc.identifier.otherROLL NO.166106108
dc.identifier.urihttps://gyan.iitg.ac.in/handle/123456789/2792
dc.language.isoen
dc.relation.ispartofseriesTH-3130
dc.titlePhytochemical Conjugated Gold Nanoparticles for the Treatment of Triple-Negative Breast Cancer
dc.typeThesis
Files
Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
Abstract-TH-3130_166106108.pdf
Size:
113 KB
Format:
Adobe Portable Document Format
Description:
ABSTRACT
Download
No Thumbnail Available
Name:
TH-3130_166106108.pdf
Size:
2.67 MB
Format:
Adobe Portable Document Format
Description:
THESIS
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
PhD Theses (Biosciences and Bioengineering)