Production purification and characterization of glutaminase-free L-asparaginase from pectobacterium carotovorum MTCC 1428
| dc.contributor.author | Kumar, Sanjay | |
| dc.date.accessioned | 2015-09-16T05:25:21Z | |
| dc.date.accessioned | 2023-10-19T11:07:45Z | |
| dc.date.available | 2015-09-16T05:25:21Z | |
| dc.date.available | 2023-10-19T11:07:45Z | |
| dc.date.issued | 2010 | |
| dc.description | Supervisor: V Venkata Dasu and K Pakshirajan | en_US |
| dc.description.abstract | Bacterial L-asparaginase has been widely used as a therapeutic agent in the treatment of certain kinds of cancer, mainly in treatment of acute lymphoblastic leukemia. Moreover, L-asparaginase is used in food industry for the production of acrylamide free food, as model enzyme for development of new drug delivery system and L-asparagine biosensor for diagnosis of leukemia. The various side effects of this drug are mainly due to the presence of partial glutaminase activity of L-asparaginase. Hence, glutaminase-free L-asparaginase is highly desirable for its successful application. Among the tested microorganism, Pectobacterium carotovorum MTCC 1428 has the ability to produce novel glutaminase-free L-asparaginase. Hence, further studies were carried out using P. carotovorum MTCC 1428. Localization, production, purification and characterization of glutaminase-free L-asparaginase have been performed by P. carotovorum MTCC 1428. Production of glutaminase-free L-asparaginase was performed both in the shake flask and bioreactor (batch and fed-batch). Furthermore, Kinetic models were developed for dual substrate growth, substrates utilization and L-asparaginase production by P. carotovorum MTCC 1428 in batch bioreactor. The localization of L-asparaginase was carried out using cell fractionation techniques. The specific activity of L-asparaginase was found to be 85 and 77% in the cytoplasm of Pectobacterium carotovorum MTCC 1428 that was grown on medium containing L-asparagine, and a combination of both L-asparagine and glucose, respectively. Among the carbon sources tested, L-asparagine and a combination of both L-asparagine and glucose were found to be the most suitable carbon sources for maximum production of L-asparaginase. Yeast extract, L-asparagine and peptone showed significant effect on the production of L-asparaginase. L-asparagine was used as an essential carbon source for the maximum production of L-asparaginase by P. carotovorum MTCC 1428......... | en_US |
| dc.identifier.other | ROLL NO.0510609 | |
| dc.identifier.uri | https://gyan.iitg.ac.in/handle/123456789/83 | |
| dc.language.iso | en | en_US |
| dc.relation.ispartofseries | TH-0959; | |
| dc.subject | BIOSCIENCES AND BIOENGINEERING | en_US |
| dc.title | Production purification and characterization of glutaminase-free L-asparaginase from pectobacterium carotovorum MTCC 1428 | en_US |
| dc.type | Thesis | en_US |