Studies on dihydrolipoamide dehydrogenase of Leishmania donovani and itsbiochemical functions

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dc.contributor.author Chiranjivi, Adarsh Kumar
dc.date.accessioned 2021-06-18T06:34:03Z
dc.date.available 2021-06-18T06:34:03Z
dc.date.issued 2019
dc.identifier.other ROLL NO.146106019
dc.identifier.uri http://gyan.iitg.ernet.in/handle/123456789/1850
dc.description Supervisors: Vikash Kumar Dubey and Pranjal Chandra en_US
dc.description.abstract Our whole work provides clear evidence about the existence of two different variants of LdDLDH enzyme which has a difference in catalytic activity. LdDLDH_Variant2 showed high physiological catalytic activity compare to LdDLDH_Variant1 during biochemical studies. These enzymes belong to the flavo group that was confirmed by estimation of FAD (flavo compound) cofactor by HPLC analysis. Other than a physiological activity such as the conversion of DLD into LA (natural substrate), it also performs a function like diaphorases. LdDLDH enzyme has also the ability to transfer an electron to any electro accepting compound which forms radical. LdDLDH_Variant1 showed greater diaphorase activity upon LdLDH_Variant2. In the search of the reason behind less activity of LdDLDH _Variant1, mutational studies were performed. Out of five mutations (C15T, C38G, A48I, D49G, and A54I), Cys-15 may be one of the members of disulfide bridge former. In flavo group of enzyme, disulfide bridge at the active site utilized in the catalytic process. All other mutated amino acids including Cys-15 having a role in the catalytic activity which was also confirmed by fluorescence study. GFP-based localization study of LdDLDH variants revealed that LdDLDH_Variant1 present in more than one compartment includes the nucleus, kinetoplast, and mitochondria whereas another variant localizes in cytoplasmic face of the plasma membrane. en_US
dc.language.iso en en_US
dc.relation.ispartofseries TH-2407;
dc.subject BIOSCIENCES AND BIOENGINEERING en_US
dc.title Studies on dihydrolipoamide dehydrogenase of Leishmania donovani and itsbiochemical functions en_US
dc.type Thesis en_US


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